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. 2013 Sep 23;2013:276808. doi: 10.1155/2013/276808

Figure 1.

Figure 1

(a) A representative scheme of frataxin isoform cDNAs and primer sequences. Frataxin isoform 1 (FXN-1) and isoform 2 (FXN-2) share the left primer sequence, while the right primer was specific for each isoform. Frataxin isoform 3 (FXN-3) right primer sequence was designed in the region downstream the 702 base pair. (b) Enzymatic digestion of FXN-1 and FXN-2 PCR products. A representative polyacrylamide gel electrophoresis (10%) of FXN-1 and FXN-2 PCR products digested or undigested with BstNI restriction enzyme. Lane 1: DNA ladder; lane 2: undigested FXN-1 PCR product; Lane 3: BstNI digested FXN-1 PCR product; lane 4: undigested FXN-2 PCR product; lane 5: BstNI digested-FXN-2 PCR product.