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Kinetic parameters of wild-type Sp SPT and mutants.
| Enzyme | kcat × 10−2
(s−1) |
Km
L-ser
(mM) |
Km
PCoA
(μM) |
kcat/Km
L-ser
(M−1 s−1) |
kcat/Km
PCoA
(M−1 s−1) |
Kd
L-ser
(mM) |
|---|---|---|---|---|---|---|
| SPT WT | 114 ± 2.0 | 1.6 ± 0.09 | 35.6 ± 2.0 | 712 | 32022 | 1.1 ± 0.1 |
| SPT V246M | 44 ± 1.2 | 2.5 ± 0.2 | 128.0 ± 10.0 | 176 | 3437 | 1.5 ± 0.1 |
| SPT G385F | 41 ± 2.0 | 3.0 ± 0.5 | 52.2 ± 8.0 | 136 | 7854 | 4.7 ± 0.1 |
| SPT G268V | — | — | — | — | — | — |
