Expressions of M2 and M3 muscarinic receptors in the bladder from the control, sham-operated, and crushing injury on nerve bundles from the MPG to the bladder group at one, two, and four weeks after injury (A); Relative protein expression of M2 and M3 muscarinic receptors of the bladder. The number of rats analyzed at each of one, two, and four weeks after injury was five in the control group, five in the sham-operated group, and 10 in the crushing injury on nerve bundles from MPG group (B). Results are expressed as mean ± standard deviation of the mean. * p < 0.05 compared with the control group, ** p < 0.05 compared with the sham-operated group.