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. 2013 Aug 27;14(9):17536–17552. doi: 10.3390/ijms140917536

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Histology and immunohistochemistry (IHC) of HCC and surrounding non-tumor liver parenchyma. (A) HCC section showed a higher numbers of CD31-positive endothelial cells (deep brown color) by IHC; (B) The section of the surrounding non-tumor region of liver parenchyma shows low CD31-positive endothelial cells by IHC; (C) Representative CT perfusion parameter (total BF) and average microvessel density (MVD), estimated by counting CD31-positive microvessels, did not show any significant trend. Microvessels were counted on a 200× field (0.62 mm²).