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. 2013 Sep 3;14(9):17958–17971. doi: 10.3390/ijms140917958

Figure 2.

Figure 2

Murine PlGF siRNA reduces PlGF expression of S3T3 fibroblasts. (A) Co-culturing S3T3 fibroblasts with prostate cancer cells increases S3T3 PlGF expression. * significantly different from S3T3 alone, p < 0.05; (B) Transfection with siRNA targeting murine PlGF efficiently reduces endogenous S3T3 fibroblast PlGF expression 24 h following transfection compared to controls transfected with scrambled siRNA. Expression levels are expressed as percentage of corresponding control values (mean ± SD). * significantly different from control, p < 0.001; (C) In co-cultures of PC-3 with S3T3 fibroblasts transfected with scrambled siRNA, treatment with siRNA targeting murine PlGF reduces murine PlGF expression on the mRNA level 24 h post-transfection as assessed by real time RT-PCR (left panel). * significantly different from control, p < 0.001. Quantification of Western blots representatively shown with respective loading control (LC) demonstrates that murine PlGF protein is reduced 24 h post-transfection in co-cultures of PC-3 with S3T3 (right panel). Expression levels are expressed as percentage of corresponding control values (mean ± SD). * significantly different from control, p = 0.003. (D) Co-culturing S3T3 fibroblasts with prostate cancer cells increases the number of Ki-67 positive PC-3 nuclei when compared PC-3 cells alone. Treatment of PC-3/S3T3 co-cultures with siRNA targeting murine PlGF significantly reduces the number of Ki-67 positive PC-3 nuclei when compared to scrambled controls or PlGF siRNA co-cultures supplemented with 5 ng/mL murine PlGF. Proliferation is expressed as percentage of corresponding control values (mean ± SD). * significantly different from PC-3 cells alone p < 0.05; † significantly different from controls and PlGF supplemented co-cultures, p < 0.05.