Figure 4.

The effect of ziyuglycoside II on expressions of p53 and p21. Cells were incubated with various concentrations of ziyuglycoside II for 24 h and the expressions of p53 and p21 were assessed by Western blot analysis. (Upper panel) Immunobloting of p53 and p21, while β-actin was probed as the protein loading control; (Lower panel): Densitometry analysis of p53 and p21 protein expression. All data were expressed as mean ± SD of three experiments and each experiment included triplicate repeats. * p < 0.05, ** p < 0.01 vs. control.