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. 2013 Sep 3;14(9):18041–18055. doi: 10.3390/ijms140918041

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

The effect of ziyuglycoside II on expressions of apoptosis related proteins. Cells were incubated with various concentrations of ziyuglycoside II for 24 h and then the expressions of Bax, Bcl-2 and cytochrome c were assessed by western blot analysis. (A) Protein expression of Bax and Bcl-2: Upper panel: immunobloting of Bax and Bcl-2, while β-actin was probed as the protein loading control; Lower panel: densitometry analysis of Bax, Bcl-2 and Bax/Bcl-2 ratio. All data were expressed as mean ± SD of three experiments and each experiment included triplicate repeats. * p < 0.05, ** p < 0.01 vs. control; (B) Immunobloting of mito-cytochrome c and cyto-cytochrome c. All data were representative of three independent experiments.