Figure 7.
The effect of ziyuglycoside II on the activity and protein cleavage of caspase-3 and caspase-9. Cells were incubated with various concentrations of ziyuglycoside II for 24 h and then further analyzed for the activity and protein cleavage of caspase-3 and caspase-9. (A) The activity of caspase-3 and caspase-9 were determined as described in Materials and Methods. All data were expressed as mean ± SD of three experiments and each experiment included triplicate repeats. * p < 0.05, ** p < 0.01 vs. control; (B) The cleavage of caspase-3 and caspase-9 were assessed by western blot analysis. All data were representative of three independent experiments.