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Time course of enzyme activity and expression of Cel6A during cultivation of Streptomyces lividans/pZRJ362-cel6A-(His)6. (A) Endoglucanase activity; (B) Expression of Cel6A-(His)6; (C) Total protein; (D) Specific activity. Endoglucanase activity (U/mL) was assayed using CMC-Na as substrate. The specific activity of supernatant was calculated as U/mg total protein. Total protein concentration was measured by Bio-Rad Protein Assay kit (Hercules, CA, USA).
