Figure 1.

Increased oxygen consumption in islets <150 μm in response to glucose. (a) Oxygen consumption rate (OCR) of isolated islets <150 μm or >150 μm after glucose stimulation from 2.8 mM to 20 mM; (b) Elevated insulin secretion of isolated islets after stimulation with glucose and palmitate. Islets of B6.V-Lep^ob mice were treated with the indicated glucose concentrations in the absence or presence of 0.5 mM palmitate (PA) in Krebs-Ringer buffer for 1 h. Insulin was measured by an ELISA and normalized to islet DNA content; (c) Time course of oxygen consumption rate (OCR) after application of different stimuli (dashed lines) at a low glucose concentration (2.8 mM); (d) Time course of OCR during elevation of glucose from 2.8 mM to 20 mM and subsequent addition of further stimuli (dashed lines). Values represent means of 5–10 independent experiments with islets obtained from different mice ± SEM; *p < 0.05; ^#p < 0.01.