Figure 4.

Effect of mTOR inhibitors on cell growth. G-415 (A) and TGBC-2TKB (B) cells were treated with LY294002, rapamycin, RAD001, and AZD8055 at shown concentrations.

Notes: Cell viability was measured after 24, 48, and 72 hours of treatment using CellTiter 96® AQueous One Solution Reagent assay. Viable cells are expressed as the percentage of control (untreated cells; 0.01% dimethylsulfoxide). Values represent the mean ± standard error of the mean of at least three independent experiments in triplicate. *P < 0.05; **P < 0.01, ***P < 0.001 versus control.

Abbreviations: DMSO, dimethyl sulfoxide; mTOR, mammalian target of rapamycin.