Figure 5.

Effect of rapamycin, RAD001, and AZD8055 on cell migration.

Notes: Assays were performed using 24-well Transwell™ plates containing polycarbonate filters with an 8 μm pore size. Before seeding, G-415 and TGBC-2TKB cells were exposed to LY294002 (10 μM), rapamycin (50 nM), RAD001 (1 nM), and AZD8055 (25 nM) for 1 hour. Dimethylsulfoxide 0.1% was used as the control. Cells were counted after 24 hours using an optic microscope in 16 randomly selected fields. Results were obtained from three separate experiments and are expressed as the mean ± standard error of the mean. ***P < 0.001 compared with control.

Abbreviation: DMSO, dimethyl sulfoxide.