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. 2013 Oct 10;9(10):e1003807. doi: 10.1371/journal.pgen.1003807

Figure 5. The strain-specific difference in Itgal message level is determined by cis-acting elements at the Civq1 locus.

Figure 5

A. mRNA levels of the canonical isoform of Itgal (Itgal-002) in adult cortex from B6, BALB/c, SWR, and CSS7 strains as determined by qRT-PCR. Each bar represents mean ± SEM. ** P<0.001 vs. B6. B. The allele-specific expression ratio of Itgal transcript (Itgal-002) levels in F1 (B6×BALB) mice. A coding SNP (rs107634043) in exon 30 was used to monitor the B6-allele and BALB/c-allele transcripts of Itgal in macrophages (MΦ), endothelial cells (EC), P1 and the adult cerebral cortices. The level of the B6-specific Itgal transcript was approximately 6-fold higher than BALB/c-transcript in adult cortex. Each bar represents the allele-specific expression ratio (B6/BALB) averaged for three F1 animals. C. Surface expression of CD11a (ITGAL) on CD11a+ brain immune cells. CD45+ CD11a+ brain cells from BALB/c mice express significantly less surface CD11a as assessed by flow cytometric staining than B6 CD45+ CD11a+ brain cells (** P<0.001).