Skip to main content
. 2013 Oct 10;9(10):e1003843. doi: 10.1371/journal.pgen.1003843

Figure 1. A DopEcR hypomorphic allele and the DopEcR-Gal4 expression pattern.

Figure 1

(A) Schematic representation of the Drosophila DopEcR gene. DopEcR contains 5 exons and spans a 12.7 kb genomic region in the 64B2-64B3 cytological interval (Flybase). The DopEcR exons are represented by boxes, and the coding regions are indicated with purple color. A green arrow indicates the direction of transcription of DopEcR. The DopEcR locus is completely uncovered by deficiency Df(3L)ED4341. The PBac(PB)c02142 (DopEcRPB1) allele harbors an insertion of the piggyBac transposable element within the second intron. The insertion site is indicated as a red arrow with an inverted triangle. (B) DopEcR transcript levels in DopEcRPB1 homozygotes and trans-heterozygotes, as assessed by RT-PCR analysis. (C–G) Expression of the GFP reporter gene (UAS-CD4-tdGFP [70]) driven by DopEcR-Gal4 (green) and synaptic neuropil labeled with the nc82 antibody against presynaptic marker protein Bruchpilot (magenta). Anterior view of the adult brain displaying DopEcR-Gal4 expression pattern (C) and the nc82 staining (E). A merged image is shown in (D). (F) Adult brain with the thoracicoabdominal ganglion (ventral view). (G) Dorsal view of the thoracicoabdominal ganglion. Scale bars represent 100 µm. ABD: abdominal ganglion; AL: antennal lobe; AMMC: antennal mechanosensory and motor center; MB: mushroom body; OL: optic lobe; SEG: subesophageal ganglion; T1: prothoracic ganglion; T2: metathoracic ganglion; T3: mesothoracic ganglion; VLP: ventrolateral protocerebrum; wing: wing neuropil.