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. 2013 Oct 10;8(10):e77015. doi: 10.1371/journal.pone.0077015

Figure 1. Temporal dynamics of TNFA and BCL3 gene transcription in TNFα treated HT1080 cells.

Figure 1

HT1080 cells were treated with TNFα and signalling through NF-κB monitored. Expression of TNFA and BCL3 was assessed using qRT-PCR (A; n=3) to measure fold-changes in mRNA (relative to t=0) and shown to be dependent on nuclear translocation of NF-κB using the inhibitor SN50 (B; n=3). Transient over expression of BCL-3 from a constitutive promoter significantly reduced TNFA transcription (C; n=3) and siRNA-induced depletion of BCL-3 (inset) prior to TNFα treatment significantly increased TNFA transcription (D; 3h time points are shown; n=3). Temporal changes in BCL-3 occupancy at a distal (-869) κB site in the TNFA promoter (E; n=3) correlated with increased expression of BCL-3 (F), which though present throughout HT1080 cells was enriched in nuclei (G; 3h time-point shown). Error bars show standard deviation: *P<0.05; **P<0.01.