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. 2013 Oct 10;8(10):e75917. doi: 10.1371/journal.pone.0075917

Figure 7. GCN2 programming of PPARγ2 during perinatal development is associated with histone 3 trimethylation.

Figure 7

(A). ChIP assay of H3K4me3, H3K9me3 and H3K27me3 on Pparγ2 promoters in livers of 3 weeks old Gcn2 KO (KO) and wild type (WT) mice. −2.0 kb, −0.3 kb, exon1, +0.5 kb indicated locations of primers used for PCR quantification of ChIP assays in the Pparγ2 promoter region. (Mean ± SEM, n = 4, *p<0.05 Gcn2 KO vs. WT). (B). ChIP assay of H3K4me3, H3K9me3 and H3K27me3 on Pparγ1 promoters in livers of 3 weeks old Gcn2 KO (KO) and wild type (WT) mice. −0.75 kb, −0.1 kb, +0.5 kb indicated locations of primers used for PCR quantification of ChIP assays in the Pparγ1 promoter region. (Mean ± SEM, n = 4). (C). ChIP assay of H3K4me3, H3K9me3 and H3K27me3 on Pparγ2 promoters in livers of 8 months old Gcn2 KO (KO) and wild type (WT) mice. −2.0 kb, −0.3 kb, exon1, +0.5 kb indicated locations of primers used for PCR quantitation of ChIP assays in Pparγ2 promoter regions. (mean ± SEM, n = 4, *p<0.05 Gcn2 KO vs. WT).