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. 2013 Oct 10;8(10):e75119. doi: 10.1371/journal.pone.0075119

Figure 3. Nano-HPLC/nano-ESI-LTQ-Orbitrap-MS/MS analysis of a cross-linked peptide mixture between CaM and skMLCK peptide.

Figure 3

The reaction was conducted at 12+ with a 50-fold molar excess of BS2G for 60 min. (A) Mass spectrum (MS) obtained at an LC retention time of 70.42 min. The 4+ charged signal of a cross-linked product at m/z 783.398 is shown enlarged. (B) Fragment ion mass spectrum (CID-MS/MS). The cross-linked product comprises amino acids 14–30 of CaM (α-peptide in red) and amino acids 8–18 of skMLCK (β-peptide in blue), in which Lys-21 of CaM is connected to Lys-8 of skMLCK.