Table 1. General features identified in the genomes of the compared methanotrophs.
| Alphaproteobacteria | Gammaproteobacteria | Verrucomicrobia | ||||||
| Features | Methylocystis sp. strain SC2 | Methylocystis sp. strain Rockwell | Ms. trichosporium OB3b | Mce. silvestris BL2 | Mc. capsulatus Bath | Mmo. methanica MC09 | Mm. alcaliphilum 20Z | Ma. infernorum V4 |
| Accession number | HE956757 | AEVM00000000 | ADVE00000000 | CP001280 | AE017282 | CP002738 | FO082060 | CP000975 |
| Status | Complete | 149 contigs | 173 contigs | Complete | Complete | Complete | Complete | Complete |
| Genome size (Mb) | 3.77 | 4.6 | 4.9 | 4.3 | 3.3 | 5.05 | 4.67 | 2.2 |
| G+C content (%) | 63 | 63 | 66 | 63 | 64 | 51 | 49 | 45 |
| Total no. of CDS | 3,666 | 4,637 | 4,4721 | 4,016 | 3,120 | 4,494 | 4,083 | 2,473 |
| rRNA operons | 1 | 1 | 1 | 2 | 2 | 1 | 3 | 1 |
| No. of tRNA genes | All (47) | All (51) | All | All | All (46) | All | All (44) | All (46) |
| tRNA genes in rrn operons 2 | Ile-Ala | Ile-Ala3 | Ile-Ala4 | Ile-Ala (in both) | Ile-Ala (in both) | Ile-Ala | Ile-Ala (in all three) | Ala-Ile |
| pmoCAB1 operon | 2 | 1 | 1 | Absent | 2 | 1 | 1 | 3 |
| pmoCAB2 operon | 1 | Absent | Absent | Absent | Absent | Absent | Absent | Absent |
| Monocistronic pmoC | 3 (1 in plasmid) | 4 | 1 | Absent | 1 | Absent | - | 1 |
| sMMO-encoding operon | Absent | Absent | 1 | 1 | 1 | 1 | - | Absent |
| pxmABC operon | Absent | Absent | Absent | Absent | Absent | Absent | - | Absent |
| Serine pathway genes | Present | Present | Present | Present | Incomplete | Absent | Absent | Present (partial) |
| RuMP pathway genes | Absent | Absent | Absent | Absent | Present | Present | Present | Absent |
| Plasmid(s) | 25 | NR6 | NR | NR | NR | NR | 17 | NR |
| CRISPRs 8 | 0 | 2 | 2 | 0 | 2 | 4 | 3 | 4 |
| No. of cas genes 9 | 0 | 2 | 5 | 0 | 10 | 7 | 18 | 8 |
1
The number of CDS predicted in the genome announcement is 4,503, while the submitted sequence actually contains 4,472 CDS.
2
tRNA genes were identified in 16S-23S spacer region of the rRNA operons.
3
rRNA operon present in contig 219 (AEVM01000005).
4
rRNA operon present in contig 00159 (NZ_ADVE01000118).
6
NR – not reported.
7
Size of plasmid: MEALZ_p (FO082061), 128 kb.
8
Abbreviation: Clustered Regularly Interspaced Short Palindromic Repeats; identified using the online tool “CRISPR finder”.
9
CRISPR-associated (cas) genes were predicted using the RAST server.