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. 2013 Oct 10;8(10):e76109. doi: 10.1371/journal.pone.0076109

Figure 2. Preparation and evaluation of dsDNAs used in SELEX-Seq.

Figure 2

A, Detection of dsDNA products of Klenow reaction with PAGE. B and C, Detection of purified dsDNA with PAGE before and after EB staining. The loading amount of dsDNA: 1, 4, 7: 1 µl; 2, 5, 8: 2 µl; 3, 6, 9: 4 µl. D and E, Detection of dsDNA with EMSA PAGE before and after EB staining. P, positive dsDNA; N, negative dsDNA; R, random dsDNA. Black arrowhead, shifted dsDNA (protein-bound dsDNA); Gray arrowhead, free dsDNA. All PAGE gels were visualized with UV transilluminator.