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Prussian blue staining of MDA-MB-231 cancer cells bound with ScFvEGFR-IONPs (A), 100 times free ScFvEGFR inhibited the binding of EGFR-positive MDA-MB-231 cancer cells (B), negative MCF-7 and MDA-MB-453 cancer cells (C and D) treated with ScFvEGFR-IONPs, and MDA-MB-231, and MDA-MB-453 treated with nonconjugated IONPs (E and F). Scale bar is 10 μm.
Abbreviations: IONPs, iron oxide nanoparticles; ScFvEGFR, single chain fragment of antibody against epidermal growth factor receptor.
