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. 2013 Oct 2;8:3757–3768. doi: 10.2147/IJN.S51353

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© Dong et al. This work is published by Dove Medical Press Ltd, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

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Tumor microvessel density images evaluated using cluster of differentiation (CD) 34 expression in the microvessel endothelium (arrow) of tumor tissue sections (original magnification × 400) from ten rabbits from each group: Lipiodol® (Guerbet, Villepinte, France) (Group A), nanoplex/Lipiodol (Group B), nanoplex-p53/Lipiodol (Group C), nanoplex-Rb/Lipiodol (Group D), and nanoplex-(p53+Rb)/Lipiodol (Group E). Immunohistochemistry analysis was undertaken with rabbit polyclonal anti-CD34 as the primary antibody, polyclonal goat anti-rabbit biotinylated antibody as the secondary antibody, and diaminobenzidine as chromogen; hematoxylin counterstaining showed high levels of protein expression in the microvascular endothelial cells of VX2 tumor from groups A, B, and C, which was downregulated in Groups D and E. Normal saline was used instead of the primary antibody for the negative control (N).