Table 3. Detectability of mosquito DNA in artificial bat feces with increasing concentrations of Aedes aegypti (by volume; 0-100 %).
% Aedes aegypti (by volume) | 0 | 5 | 10 | 15 | 20 | 25 | 100 |
---|---|---|---|---|---|---|---|
Replicate 1 | ✓ | ✓✗ | ✓✗ | ✓✗ | ✓✗ | ✓✗ | ✗ |
Replicate 2 | ✓ | ✓✗ | ✓✗ | ✓✗ | ✓✗ | ° | ✗ |
Replicate 3 | ✓ | ✓✗ | ° | ✓✗ | ✓✗ | ✓✗ | ✗ |
% of mosquito sequences in clone library | n/a | 20 | 30 | 10 | 30 | 30 | n/a |
✗ represents successful PCR amplification with a corresponding DNA sequence matching Ae. aegypti; ✓ represents successful PCR amplification with a corresponding DNA sequence matching T. molitor; ✓✗ represents mixed DNA sequence; ° represents a non-readable sequence due to excessive loss of PCR products during purification prior to sequencing.