Figure 1. Transient over-expression of Pi03192 in planta increases P. infestans virulence.

Agrobacterium was used to transiently over-express Pi03192 on one half of a leaf and empty vector on the other. Leaves were subsequently infected with P. infestans. (A) Shows a typical leaf with larger lesions on the half of the leaf expressing Pi03192. (B) The leaf in (A) stained with trypan blue to show increased P. infestans mycelial growth on the half of the leaf expressing Pi03192. (C) Graph quantifying lesion area in the presence or absence of Pi03192 expression. Error bars show standard error and asterisks indicate a highly significant difference (p<0.001, t-test) (from 3 biological replicates each comprising 17 inoculations per treatment).