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. 2013 Oct 10;9(10):e1003670. doi: 10.1371/journal.ppat.1003670

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© 2013 McLellan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) BiFC images showing that YFP fluorescence is reconstituted at the ER when YC-StNTP1 and YC-StNTP2 are co-expressed with YN-Pi03192, but not with YN-Avr2. The arrowheads indicated the ER membrane surrounding the nucleus. Scale bars indicate 35 µm. (B) Graph shows relative fluorescence quantified using a fluorimeter. Error bars show standard error and asterisks indicate a very highly significant difference (p<0.001, t-test) in fluorescence of YC-StNTP1 or YC-StNTP2 co-expressed with YN-Pi03192, compared to co-expression with YN-Avr2. (C) Immunoblots showing stability of full length YN-Pi03192 and YN-Avr2 (myc antibody) fusions and YC-StNTP1 and YC-StNTP2 (HA antibody) fusions.