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. 2013 Oct 10;9(10):e1003670. doi: 10.1371/journal.ppat.1003670

Figure 6. Silencing of Pi03192 compromises pathogenicity which can be restored in TRV:NTP plants.

Figure 6

(A) Graph shows expression levels of Pi03192 are severely down regulated in 03192_IR compared to WT (strain 88069) while three other known P. infestans avirulences are unaffected. (B) Graph shows 03192_IR is significantly less able to infect both potato and N. benthamiana compared to WT (two tailed t-test p<0.005,n = 8). Images show symptoms of WT and 03192_IR infection on (C) potato and (D) N. benthamiana. (E) Graph shows that in planta expression of Pi03192 is able to restore virulence of 03192_IR to WT levels (strain 88069 inoculated with transient expression of empty vector control; vec) (no significant difference with one way anova p = 0.83, n = 55). In contrast, colonisation by 03192_IR with expression of the empty vector control (vec) is significantly reduced (one way anova p<0.05, n = 73). (F) Graph shows significant increases in lesion diameter for 03192_IR on each TRV:NTP silenced line compared to the TRV:GFP control (two tailed t-test p<0.05, n = 30). (G) Images show 03192_IR symptoms on TRV:NTP silenced plants and the TRV:GFP control. Error bars are standard error.