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. 2013 Oct 10;9(10):e1003670. doi: 10.1371/journal.ppat.1003670

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© 2013 McLellan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Confocal images of localisation of either GFP-StNTP1 or GFP-StNTP2 co-expressing either pFlub (vec) or pFlub-03192 and treated with Media or culture filtrate, plus and minus MG132. Scale bar is 10 µm. (B) Graph shows the percentage of nuclei containing either GFP-StNTP1 or GFP-StNTP2 fluorescence with each treatment. Error bars are standard error and one-way ANOVA analysis shows that treatment with CF and MG132, when co-expressed with empty vector (vec), is statistically different from the other treatments, which are not different from each other. Significance is indicated by p-value and asterisks. (C) Immunoblots show the stability of GFP-StNTP1 and GFP-StNTP2 with different treatments as indicated, probed with a specific GFP antibody. PS is Ponceau stain.