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. 2013 Oct 10;8(10):e77195. doi: 10.1371/journal.pone.0077195

Figure 5. The effect of incubation temperature on extracellular (A+C) and intracellular (B+D) Alanine concentration.

Figure 5

The concentration of alanine in media (A,C) and lysed cells (B,D) for CHOK1 (A+B) and CHOS (C+D) is plotted as percentage of the maximum of each metabolite recorded within the data set. Shift in culture temperatures is denoted by the wide dashed line, and recovered temperature with the short dashed line. Legend: • 37 °C, ■ 27 °C shift, ▲ 10 °C shift, □ 27 °C recover, △ 10 °C recover.