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. 2013 Oct 10;8(10):e77097. doi: 10.1371/journal.pone.0077097

Figure 4. Knockdown of IDHc expression in dispersed rat islet cells increases glucose-induced hormone release.

Figure 4

ScrAB and siIDH#2 (siIDHc) were co-transfected with human growth hormone (hGH) plasmid in dispersed rat islet cells. Cells were cultured for 48 h prior to the experiment. hGH release was measured in cells incubated at 2 or 16 mM glucose (G) or 2 mM glucose plus 35 mM KCl. hGH release was normalized by hGH cellular content and is expressed as fold increase over the 2 mM glucose condition. Data represent the mean ± SEM of three independent experiments performed in triplicate. * p<0.05 vs ScrAB under the same incubation condition by paired two-tailed Student t test.