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. 2013 Oct 10;8(10):e77189. doi: 10.1371/journal.pone.0077189

Figure 1. Identification of cancer cell specific cytotoxicity in the water extract of Ashwagandha leaves by in vitro and in vivo assays.

Figure 1

(A) Human normal and cancer cells were treated with three kinds (prepared in different ways as described in materials and methods) of water extracts, WEX-1 (ASH-WEX-1) WEX-2 (ASH-WEX-2) and WEX-3 (ASH-WEX-3). WEX-1 and WEX-2 were predominantly cytotoxic to cancer cells. WEX-3 did not show any cytotoxicity. (B and C) Human cancer and normal cells treated with serial doses of ASH-WEX and stained with crystal violet showed toxicity to cancer cells. (D) Mice fed with WEX (500 mg/Kg body weight) every alternate day did not show any loss in body weight compared to control mice. (E and F) Nude mice with HT1080 subcutaneous xenograft when fed with WEX showed significant inhibition of tumor progression as well as lung metastasis (G). ASH-WEX has been labeled as WEX in all the figures.