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. 2013 Oct 10;8(10):e77176. doi: 10.1371/journal.pone.0077176

Figure 3. Involvement of ionic channels and intracellular calcium on stimulatory effect of rT3 on 45Ca2+ uptake.

Figure 3

(A) Influence of flunarizine, (B) BAPTA-AM and (C) 9-AC on stimulatory effect of rT3 on 45Ca2+ uptake in Sertoli cells. Pre-incubation: 15 min in KRb, additional pre-incubation: 60 min with 0.1 µCi/mL of 45Ca2+ and incubation time: 60 s with 0.1 µCi/mL of 45Ca2+ in the presence or absence of flunarizine (1 µM), BAPTA-AM (50 µM) and 9-AC (1 µM) with/without rT3 (10-17 M). Means ± S.E.M. For control, n=10; rT3, n=7; flunarizine, n=8; rT3 + flunarizine, n=8; BAPTA-AM, n=8; rT3 + BAPTA-AM, n=6; 9-AC, n=6; rT3 + 9-AC, n=6. ***P < 0.001 and **p < 0.01 compared with control group; ###p < 0.001; ##p < 0.01 and #p < 0.05 compared with rT3 group.