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. 2013 Oct 10;9(10):e1003710. doi: 10.1371/journal.ppat.1003710

Figure 7. SA and TS activities of T. b. gambiense BSF and involvement in BAE activation.

Figure 7

(A) SA and (B) TS activities were measured on crude extracts of T. b. gambiense 1135 and LiTat BSF and compared with previous results on T. congolense, T. vivax and T. b. brucei BSF [21], [22]. Activities are expressed as nmol of sialic acid released or transferred per min by 109 lysed parasites. (C) Percentage of activated BAE by T. b. brucei 427 expressing heterologous TS of T. b. gambiense T. b. brucei TbgSA B and T. b. brucei TbgSA B compared to non-transfected and transfection control cell lines. (D) Effect of the TS inhibitor myricetin on BAE activation by T. b. gambiense 1135 BSF. Results are represented as percentage of activation capacity and normalized to the control (absence of myricetin). Myricetin concentration is indicated on the X-axis. Data are expressed as mean values±SD of three independent experiments. See also Fig. S5 and S6, and Table S2.