Skip to main content
. 2013 Oct 10;9(10):e1003681. doi: 10.1371/journal.ppat.1003681

Figure 3. CD161 triggering inhibits the cytokine secretion but spares the cytotoxic response of MAIT cells.

Figure 3

(A–B) MACS sorted MAIT cells were activated by Ec-infected Hela-hMR1 in presence of control, anti-MR1 (10 µg/ml), anti-NKG2D (10 µg/ml) or anti-CD161 antibody (10 µg/ml). (A) Upregulation of CD69 and CD25 was assessed by FACS and (B) the cytotoxic effect of MAIT cells by LDH release. Data are mean and SEM of two independent experiments. * indicates statistical significance for the MOI dose-response curve, while “ns” indicates the absence of effect of anti-NKG2D or anti-CD161 treatment by 2 way ANOVA. (C) MAIT cells were FACS sorted using anti-Vα7.2 and anti-IL18Rα before being stimulated with increasing doses of plastic-coated anti-CD3 (as indicated) and anti-CD28 (10 µg/ml) monoclonal antibodies in the presence of increasing doses of anti-CD161 antibody. Cytokines were quantified after 24 h stimulation. Data are mean and SEM of two independent experiments.