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. 2013 Oct 10;8(10):e77479. doi: 10.1371/journal.pone.0077479

Figure 1. Gating strategies for CMV-SP-CD4 T cells.

Figure 1

All flow cytometry plots are from a single subject of the RESTORE study. Lymphocytes were identified using forward and side scatter (Figure 1A), followed by gating on CD4+ T cells (Figure 1B). Gates for CD25+ and CD134+ cells were placed based on comparison with negative control (Figure 1C) and PHA positive control (Figure 1D) to include cells highly co-expressing CD25 and CD134. A representative example of the dynamics of responses to CMV antigens over 96 weeks of follow-up in a RESTORE subject (Figure 1E-K).