Figure 4. Biofilm formation by doxycycline-induced expression of the hyperactive STE11 ^ΔN467 allele in the wild-type strain WO-1 and the indicated mutants.

White cells of the strains were grown for 24°C in liquid medium in the absence (−) or presence (+) of doxycycline. Nonadherent cells were removed by careful washing. Two independently constructed strains were tested in each case; the untransformed parental strain WO-1 served as control. (A) Photographs of the wells of the microtiter plates. (B) Quantification of biofilm formation by crystal violet staining. Results are the means and standard deviations from three biological replicates.