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. 2013 Oct 10;9(10):e1003696. doi: 10.1371/journal.ppat.1003696

Figure 7. Induction of white-opaque switching in MTLa and MTLα derivatives of strain SC5314 after expression of CPH1 from the inducible OPT3 promoter.

Figure 7

White cells of the strains were grown for 18-BSA-YE medium at 30°C, diluted, and plated on Lee's medium with phloxin B to determine the percentage of opaque colonies (including a small number of mixed white/opaque colonies). Two independently constructed series (A and B) of MTL a and MTLα strains were tested in each case; the untransformed parental strains served as controls. The gray and black bars show the results with transformants in which the POPT3-CPH1 fusion was integrated into the OPT3-2 and OPT3-1 allele, respectively, which could be distinguished by a SpeI restricton site polymorphism [33]. Results are the means and standard deviations from three biological replicates. Only background switching frequencies were observed when the strains were grown in noninducing YPD medium (not shown).