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. 2013 Oct 10;8(10):e77493. doi: 10.1371/journal.pone.0077493

Figure 3. CCR2 deficiency reduces profibrotic chemokine production.

Figure 3

A. CCR2 deficiency inhibits MCP-1 and CXCL16 gene expression in the kidneys 7 days after UUO. The mRNA levels of MCP-1 and CXCL16 in the kidneys of WT and CCR2-KO mice are determined by real-time RT-PCR. ** P < 0.01 vs WT controls, # P < 0.05 vs WT UUO and ++ P < 0.01 vs KO UUO. n=4 per group. B. TNF-α and IFN-γ synergistically up-regulate MCP-1 and CXCL16 mRNA expression in mouse tubular epithelial cells. The mRNA levels of CXCL16 in mouse tubular epithelial cells are determined by real-time RT-PCR. ** P < 0.01 vs vehicle controls. n=5 per groups. C. CCR2 deficiency inhibits TNF-α gene expression in the kidney 7 days after UUO. The mRNA levels of TNF-α in the kidneys of WT and CCR2-KO mice are determined by real-time RT-PCR. ** P < 0.01 vs WT controls, # P < 0.05 vs WT UUO and ++ P < 0.01 vs KO UUO. n=5 per group. D. CCR2 deficiency inhibits IFN-γ gene expression in the kidneys 7 days after UUO. The mRNA levels of IFN-γ in the kidneys of WT and CCR2-KO mice are determined by real-time RT-PCR. ** P < 0.01 vs WT controls, # P < 0.05 vs WT UUO and ++ P < 0.01 vs KO UUO. n=5 per group.