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. 2013 Oct 10;8(10):e77396. doi: 10.1371/journal.pone.0077396

Figure 4. Comparison between RNA-seq and qPCR experiments of genes involved in the melanocortin system.

Figure 4

Quantitative PCR validation of pro-opiomelanocortin and other highly expressed genes involved in the processing of the prohormone found by RNA-seq in the silver eel samples: pro-opiomelanocortin (pomc), prohormone convertase 2 copy 1 (pc2a), prohormone convertase 2 copy 2 (pc2b), secretogranin II copy 1 (scg2a), secretogranin II copy 2 (scg2b), secretogranin III copy 1(scg3a), secretogranin III copy 2 (scg3b), neuroendocrine protein 7b2 copy 1(7b2a), neuroendocrine protein 7b2 copy 2 (7b2b) and carboxypeptidase e (cpe). Note that pc2b and scg2a were studied by RNA-seq only, and not investigated by qPCR due to limitations for designing primers from these genes where we only obtained partial sequences. Primer sequences are given in table S1 and details about the annotation of the genes are provided in table S4. Results are presented as means ± standard deviations (SD) on log scales for both methods, where the left y-axis represent the re-quantified RNA-seq normalized gene expression (n=4) and the right y-axis represent the expression by qPCR (n=5).