Table 1.
PCR target | Primer | Forward | Reverse |
---|---|---|---|
Genomic and cDNA sequences | TaEG-1 | CCCTTCCGTTCCCATTCCAAGC | ACATGGCATGGTGATGATACT |
Chromosomal location | TaEG-IN3 | GGATAATAAGCTCCCTGGTG | TACAATCGTAAGTGCCCTGT |
Real-time PCR | TaEG-2 | GCGGGCAACGCTGGTCTA | GGGAACATTGGCGGCACA |
Internal control | Ubiquitin | AAGGCGAAGATCCAGGACAAG | TGGATGTTGTAGTCCGCCAAG |
Actin | ACGCTTCCTCATGCTATCCTTC | ATGTCTCTGACAATTTCCCGCT |