FIGURE 2.
Klotho and sialidase stabilize TRPV5 on the plasma membrane. A and B, TIRF-M images of HEK293 cells expressing Dendra-2-TRPV5 or Dendra-2-TRPV5N358Q in both red and green channels, after 0 and 30 min. C, ratio of switched red/unswitched green fraction of HEK293 cells expressing Dendra-2-TRPV5. These cells were subjected to live-cell TIRF-M. At 0 min Dendra-2 was subjected to UV light (λ = 405 nm), resulting in a switch from green to red. D, ratio of switched red/unswitched green fraction of HEK293 cells expressing Dendra-2-TRPV5N358Q. These cells showed no increase in plasma membrane retention after glycosidase treatment. ○, control; □, klotho-treated; ●, sialidase-treated. All data are expressed as mean ± S.E.; n = 3.