FIGURE 3.

TRPV5 stabilization via klotho is N-glycan-dependent. A, biotinylation of HEK293 cells expressing TRPV5 or TRPV5^N358Q after klotho treatment, a representative blot is depicted; B, relative TRPV5 cell surface expression with or without klotho treatment (white and black bar, respectively); C, biotinylation of HEK293 cells expressing TRPV5 or TRPV5^N358Q after sialidase treatment, a representative blot is depicted; D, relative TRPV5 cell surface expression with or without sialidase treatment (white and black bar, respectively); E, ⁴⁵Ca²⁺ uptake assay of cells transfected with TRPV5 or TRPV5^N358Q after treatment with klotho, sialidase, a combination of these glycosidases, ruthenium red or left untreated. All data are presented as mean ± S.E.; n = 3; *, p < 0.05, statistically significant.