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. 2013 Aug 22;288(41):29238–29246. doi: 10.1074/jbc.M113.473520

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Galectin-3 is a candidate mediator of the klotho effect on TRPV5. A, immunohistochemistry on DCT/CNT for TRPV5 and galectin-1 shows no co-localization. B, immunoblot of CHO cell lysate demonstrated that galectin-1 was present. After RT-PCR on the cDNA of HEK293 cells, no galectin-1 was detected. C, immunohistochemistry on DCT/CNT for TRPV5 and galectin-3 demonstrate a co-localization; D, immunoblot of CHO cell lysate demonstrated that galectin-3 was expressed. After RT-PCR on the cDNA of HEK293 cells, galectin-3 was detected. E, cDNA of the DCT/CNT cells was analyzed by RT-PCR and only galectin-3 was detected. F, total kidney lysate of mouse contains both galectin-1 and galectin-3, while mouse urine only contains galectin-3.