FIGURE 7.
TRPV5 channel activity is regulated by extracellular galectin-3. A, 45Ca2+-uptake assay in cells overexpressing TRPV5 (n = 5) or TRPV5N358Q (n = 3) in the presence and absence of human galectin-3. Galectin-3 significantly increased wild type TRPV5 (white bar) but not TRPV5N358Q channel activity (white bar). All data are presented as mean ± S.E.; *, p < 0.05, statistically significant. B, 45Ca2+-uptake assay of cells overexpressing TRPV5 treated with klotho, sialidase, galectin-3, or a combination of these enzymes. No additional stimulation was detected after treatment with klotho and galectin-3. All data are presented as mean ± S.E.; n = 5; *, p < 0.05, statistically significant.