FIGURE 11.

Bar graph of the coupling energies (ΔΔG_act,interact) for the double glycine mutants of Ca_V3.2, ΔΔG_act,interact = (ΔG_act,WT + ΔG_act,double) − (ΔG_act,S4-S5 + ΔG_act,S6). As seen, the interaction energies were significantly stronger for V907G/I1013G and V907G/N1548G than for any other double mutant. Data are shown in kcal mol⁻¹. Symbols * and ** identify double mutants with interaction energies significantly larger than 1.5 kcal mol⁻¹ at p < 0.05 and p < 0.01, respectively. Numerical values are found in Table 3.