FIGURE 7.
miR-29a suppressed AR expression by promoting p53 protein level. A, real time PCR analysis of Cdc42 and p85α mRNA levels in PC-1 cells transfected with miR-29a mimics or control mimics (mean ± S.E. (error bars), n = 3). B, Western blotting analysis of AR and p53 protein levels in PC-1 cells transfected with miR-29a mimics or control mimics. C, Western blotting analysis of AR and p53 protein levels in PC-1 cells transfected with miR-29a inhibitor or control inhibitor. D, real time PCR analysis of Cdc42 and p85α mRNA levels in epididymis from transgenic mice and littermate controls (mean ± S.E. (error bars), n = 3). E, Western blotting analysis of p53 protein levels in epididymis from transgenic mice and littermate controls. F, Western blotting analysis of AR and p53 protein levels in PC-1 cells transfected with siRNAs (si) against mouse p53.