FIGURE 8.

Inhibitory effect of SNX26 on spine formation can be largely abrogated by constitutively active Cdc42 co-expression. A, cultured hippocampal neurons were co-transfected at DIV 13 with GFP or GFP-Cdc42CA and mCherry-SNX26 and fixed at DIV 14. Scale bar, 20 μm. B–E, quantification of the number of total protrusions, spines, and filopodia with expression of SNX26 or SNX26 with Cdc42CA. Spines were defined as dendritic protrusions of <5 μm in length with heads and filopodia as dendritic protrusions of <10 μm in length without heads. Data were collected from 18 to 26 dendrites of 10–18 neurons for each group in three independent experiments. ***, p < 0.001; *, p < 0.01 (Student's t test).