Skip to main content
. 2013 Aug 29;288(41):29613–29620. doi: 10.1074/jbc.M113.500967

FIGURE 2.

FIGURE 2.

BDNF increases VLDLR gene expression in hippocampal neurons. A, hippocampal neurons were stimulated with 50 ng/ml BDNF for 12 h in the absence or presence of 30 ng/ml actinomycin D to block gene transcription. β-Actin was used as a control. The increase in VLDLR was inhibited by actinomycin D. A typical immunoblot is shown, and the experiment was repeated three times. B, RNA was prepared from control and 24-h BDNF-stimulated neurons, and qPCR was performed as described under “Experimental Procedures.” Note the increase in VLDLR mRNA by BDNF. Values are means ±S.D. (n = 3). ***, p < 0.001 for BDNF versus the control. C, hippocampal neurons were transfected with the VLDLR promoter-luciferase reporter construct as described under “Experimental Procedures” and further stimulated for 24 h with 50 ng/ml BDNF. Luciferase activity was measured, and values were corrected against Renilla activity. VLDLR gene activity was increased by BDNF. The basic promoter showed no activity and is not included here. Values are means ± S.D. (n = 3). *, p < 0.05 for BDNF versus the control.