FIGURE 8.

Dose-dependent TSG-6-mediated enhancement of fl-HA binding to CD44. AKR1/CD44+ cells were incubated with 0–5 μg/ml fl-HA in the absence or presence of 0.25 μm TSG-6, and the fluorescence intensity of ∼10,000 cells was analyzed. Representative HA binding data are shown (in gray), and the no fl-HA (negative control) is depicted as a black outline. Mean fluorescence values were calculated for fl-HA incubated in the absence (dashed red line) and presence (solid black line) of TSG-6 from three independent experiments performed in triplicate and plotted (± S.E.) in the lower panel.