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Effect of glycosylation at FVIII position 2159 on VWF binding. FVIII-WT (A) or FVIII-R2159N (B) were passed over immobilized VWF on a CM5 sensor chip as described under “Experimental Procedures.” Inset, binding response at equilibrium (Req) was plotted as a function of the FVIII-WT (●) and FVIII-R2159N (○) concentration.
