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Effect of glycosylation at FVIII position 2159 on LRP binding. FVIII-WT (A) or FVIII-R2159N (B) were immobilized via the anti-C2 domain antibody EL14 on a CM5 sensor chip, and LRP cluster II was passed over as described under “Experimental Procedures.” Inset, binding response at equilibrium (Req) was plotted as a function of the FVIII-WT (●) and FVIII-R2159N (○) concentration.
