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Effect of glycosylation at FVIII position 2159 on cellular uptake. FVIII-WT and FVIII-R2159N were incubated with U87-MG cells (A) and dendritic cells (B) for 30 min at 37 °C, and cellular uptake was analyzed as described under “Experimental Procedures.” ●, FVIII-WT; ○, FVIII-R2159N. Data are shown as mean ± S.D. from three experiments.
